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thp1 asc gfp reporter cells  (InvivoGen)


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    InvivoGen thp1 asc gfp reporter cells
    Thp1 Asc Gfp Reporter Cells, supplied by InvivoGen, used in various techniques. Bioz Stars score: 95/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thp1+asc+gfp+reporter+cells/THP1-ASC-GFP+Cells/pm41457250-72-0-4
    Average 95 stars, based on 75 article reviews
    thp1 asc gfp reporter cells - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Transfection:

    Article Title: The Glycolytic Metabolite Methylglyoxal Covalently Inactivates the NLRP3 Inflammasome
    Article Snippet: Methylglyoxal (MGO) was obtained from Acros Organics (40 wt% solution in water, Cat. No. 175791000) and freshly diluted in cell culture media prior to administration at the indicated concentrations. .. WT THP1 cells, THP1-ASC-GFP Reporter cells, and HEK-BlueTM IL-1β cells were obtained from Invivogen and maintained according to Invivogen protocols. siRNA transfections were performed using LipofectamineTM RNAiMAX Transfection Reagent (Invitrogen, 13778075) following manufacturer’s protocol with 9 μL of RNAiMAX reagent and 3 μL of 10 μM siRNA in 300 μL of Optimem per 2 million cells in a 6 well dish. ..

    other:

    Article Title: The glycolytic metabolite methylglyoxal covalently inactivates the NLRP3 inflammasome.
    Article Snippet: WTTHP1 cells, THP1-ASC-GFP Reporter cells, and HEK-Blue IL-1b cells were obtained from Invivogen andmaintained according to Invivogen protocols.

    Article Title: The glycolytic metabolite methylglyoxal covalently inactivates the NLRP3 inflammasome
    Article Snippet: WT THP1 cells, THP1-ASC-GFP Reporter cells, and HEK-Blue IL-1β cells were obtained from Invivogen and maintained according to Invivogen protocols.

    Cell Culture:

    Article Title: Cryogenic microcarrier-assisted stem cell storage (Cryo-MASCS): a preservation method enabling scalable culture of human mesenchymal stem cells
    Article Snippet: MSCs were washed with 1X PBS and fixed with 4% paraformaldehyde (PFA) solution (SC-281692, Santa Cruz Biotechnology) for 15 min. Cytoskeletal staining was performed using ActinRedTM 555 ReadyProbes reagent ( R37112 , Invitrogen) at the manufacturer-recommended dilution of 2 drops per mL for 30 min. Fluorescent images were subsequently taken at 20X (MRH00201, Nikon) magnification using a Keyence BZ-X800 fluorescence microscope. .. THP1-ASC-GFP reporter cells (thp-ascgfp, InvivoGen) were cultured in RPMI 1640 medium (11875093, Thermo Fisher Scientific) supplemented with 2 mM L-glutamine, 25 mM HEPES (H0687, Sigma-Aldrich), 10% heat-inactivated FBS, 100 μg/mL P/S, and 50 μg/mL NormocinTM (Ant-nr-05, InvivoGen). ..

    Article Title: Cryogenic microcarrier-assisted stem cell storage (Cryo-MASCS): a preservation method enabling scalable culture of human mesenchymal stem cells.
    Article Snippet: MSCs were washed with 1X PBS and fixed with 4% paraformaldehyde (PFA) solution (SC-281692, Santa Cruz Biotechnology) for 15 min. Cytoskeletal staining was performed using ActinRedTM 555 ReadyProbes reagent (R37112, Invitrogen) at the manufacturer-recommended dilution of 2 drops per mL for 30 min. Fluorescent images were subsequently taken at 20X (MRH00201, Nikon) magnification using a Keyence BZ-X800 fluorescence microscope. .. THP1-ASC-GFP reporter cells (thp-ascgfp, InvivoGen) were cultured in RPMI 1640 medium (11875093, Thermo Fisher Scientific) supplemented with 2 mM L-glutamine, 25 mM HEPES (H0687, Sigma-Aldrich), 10% heat-inactivated FBS, 100 μg/mL P/S, and 50 μg/mL NormocinTM (Ant-nr-05, InvivoGen). ..

    Article Title: Covalent targeting as a common mechanism for inhibiting NLRP3 inflammasome assembly
    Article Snippet: .. Cell Culture and High Throughput Screening: THP1-ASC-GFP Reporter cells, WT THP-1 cells, and HEK-Blue TM IL-1β cells were obtained from Invivogen and maintained according to protocols supplied by Invivogen. ..

    High Throughput Screening Assay:

    Article Title: Covalent targeting as a common mechanism for inhibiting NLRP3 inflammasome assembly
    Article Snippet: .. Cell Culture and High Throughput Screening: THP1-ASC-GFP Reporter cells, WT THP-1 cells, and HEK-Blue TM IL-1β cells were obtained from Invivogen and maintained according to protocols supplied by Invivogen. ..



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